Combined use of .beta.-galactosidase and sialyltransferase coupled with in situ regeneration of CMP-sialic acid for one pot synthesis of oligosaccharides
US5374541A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | May 4, 1993 |
| Grant date | Dec 20, 1994 |
| Priority date | — |
| Expiry date | May 4, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y306/01001
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A single reaction vessel process for the synthesis of a sialylated galactoside is disclosed. The synthesis utilizes a .beta.-galactosidase to catalyze the reaction of a galactose-containing substrate and an acceptor to form a new galactosyl glycoside that is then sialylated using a cyclic multienzyme synthesis system to form CMP-sialic acid that sialylates the formed galactosyl glycoside in the presence of an .alpha.-sialyltransferase. The value of K.sub.m /V.sub.max for the formed galactosyl glycoside as a substrate for the .alpha.-sialyltransferase is less than one-third the K.sub.m /V.sub.max value for the galactose-containing substrate for that .alpha.-sialyltransferase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.