Method for determining the relative amount of a viral nucleic acid segment in a sample by the polymerase chain reaction
US5389512A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 20, 1994 |
| Grant date | Feb 14, 1995 |
| Priority date | — |
| Expiry date | May 20, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/703
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for determining the relative amount of a nucleic acid segment in a sample by the polymerase chain reaction. The method involves the simultaneous amplification of the nucleic acid segment and a second nucleic acid segment present in the sample. The amount of amplified DNA from each segment is determined and compared to standard curves to determine the amount of the nucleic acid segment present in the sample before amplification expressed as a ratio of first segment to second segment. The method is especially preferred for determining the viral load, or copies of virus genome/host cell, in a sample of cells from an individual infected with a virus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.