Method of purifying recombinant proteins from corresponding host cell proteins
US5411864A · kind A · utility
3Cited by
1References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Nov 5, 1987 |
| Grant date | May 2, 1995 |
| Priority date | — |
| Expiry date | Nov 5, 2007 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y304/21069
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for purifying a recombinant protein, e.g. human tissue plasminogen activator (t-PA) from a corresponding endogenous protein of the host cell, e.g. Chinese hamster t-PA, is disclosed. The method comprises contacting a fluid containing the recombinant protein with antibodies specifically binding the corresponding endogenous protein. Monoclonal antibodies directed against and an assay for Chinese hamster t-PA are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.