Recombinant library screening methods
US5427908A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 1990 |
| Grant date | Jun 27, 1995 |
| Priority date | — |
| Expiry date | May 1, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1037
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Nucleotide sequences encoding proteins of interest are isolated from DNA libraries using bacteriophage to link the protein to the sequence which encodes it. DNA libraries are prepared from cells encoding the protein of interest and inserted into or adjacent to a coat protein of a bacteriophage vector, or into a sequence encoding a protein which may be linked by means of a ligand to a phage coat protein. By employing affinity purification techniques the phage particles containing sequences encoding the desired protein may be selected and the desired nucleotide sequences obtained therefrom. Thus, for example, novel proteins such as monoclonal antibodies may be produced and conventional hybridoma technology avoided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.