Purification of insulin-like growth factor
US5446024A · kind A · utility
15Cited by
8References
19Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 17, 1993 |
| Grant date | Aug 29, 1995 |
| Priority date | — |
| Expiry date | Dec 17, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for purifying IGF-I from variants of IGF-I is provided. In this process a mixture containing IGF-I and its variants is loaded onto a reversed-phase liquid chromatography column and the IGF-I is eluted from the column with a buffer at a pH of about 6-8. The buffer contains an alcoholic or polar aprotic solvent at a concentration of about 20-30% (v/v). This process can be preceded by a hydrophobic-interaction chromatography step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.