Method for purifying polypeptides
US5451660A · kind A · utility
30Cited by
22References
25Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 13, 1993 |
| Grant date | Sep 19, 1995 |
| Priority date | — |
| Expiry date | Dec 13, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/65
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for selectively separating a polypeptide of interest from components of differing hydrophobicity in a mixture comprising the steps of: PA1 (a) passing the mixture through underivatized silica particles such that the polypeptide adheres to the silica particles; PA1 (b) washing the silica particles to remove impurities; and PA1 (c) eluting the polypeptide from the silica particles with a buffer comprising an alcoholic or polar aprotic solvent and an alkaline earth, an alkali metal, or an inorganic ammonium salt.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.