Mature human fibroblast interferon
US5460811A · kind A · utility
26Cited by
7References
6Claims
0Family size
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Key dates
| Filing date | Jun 12, 1989 |
| Grant date | Oct 24, 1995 |
| Priority date | — |
| Expiry date | Jun 12, 2009 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A cDNA library is constructed using mRNA from human fibroblasts induced with poly(I):poly(C). A bacterial clone containing fibroblast interferon cDNA sequences identified by hybridization to a cDNA probe synthesized using deoxyoligonucleotide primers which hybridize to fibroblast interferon mRNA specifically. Expression plasmids are constructed which permit the synthesis in E. coli of 8.times.10.sup.7 units of human fibroblast interferon per liter of culture. The bacterially produced fibroblast interferon is indistinguishable from authentic human fibroblast interferon by several critieria.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.