Method for making a monoclonal antibody, monoclonal antibodies to .alpha. P D

US5468468A · kind A · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• William LaRochelle · Madison, US
• Jacalyn H. Pierce · Bethesda, US
• Roy A. Jensen · Westminster, US
• Stuart A. Aaronson · Great Falls, US

Key dates

• Filing date: Jun 25, 1993
• Grant date: Nov 21, 1995
• Expiry date: Jun 25, 2013

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/71
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Potent neutralizing monoclonal antibodies to the human .alpha. PDGF receptor (.alpha. PDGFR) and fragments thereof are described. These monoclonal antibodies specifically bind to an epitope on .alpha. PDGFR, inhibits PDGF binding with PDGF, antagonizes PDGF, and does not bind .beta. PDGFR receptor. A hybridoma cell line producing such a monoclonal antibody, methods of in vivo imaging of a pathological conditions and methods of inhibiting the growth of a neoplasia expressing .alpha. PDGFR, which use these monoclonal antibodies are also described. In vitro assays for detecting the presence of .alpha. PDGFR and for evaluating the binding affinity of a test compound are also described.