Non-enzymatic ligation of oligonucleotides
US5476930A · kind A · utility
173Cited by
1References
7Claims
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Key dates
| Filing date | Jan 23, 1995 |
| Grant date | Dec 19, 1995 |
| Priority date | — |
| Expiry date | Jan 23, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6813
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A process for non-enzymatic convalent ligation of two oligonucleotides having the structure shown below: ##STR1## The spontaneous reaction of these above oligonucleotides produces a thiophosphorylacetylamino linkage as shown below: ##STR2## When carried out in the presence of a template, this reaction gives 90% yields within 20 minutes at micromolar concentrations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.