Methods for producing probes capable of distinguishing DNA from related sources
US5501964A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 9, 1993 |
| Grant date | Mar 26, 1996 |
| Priority date | — |
| Expiry date | Nov 9, 2013 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6855
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methodology is provided for developing probes for identifying sequence differences between two related DNA populations, sets of DNA fragments or collections of restriction-endonuclease-cleaved DNA or cDNA. The method employs an initial stage to obtain a representation of both DNA populations, namely using the PCR to produce relatively short fragments, referred to as amplicons. Tester amplicons containing target DNA, sequences of interest, are ligated to adaptors and mixed with excess driver amplicons under melting and annealing conditions, followed by PCR amplification. The process may be repeated so as to greatly enrich the target DNA. Optionally, the target DNA may then be cloned and the DNA used as probes.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.