Assay for Ribozyme target site
US5525468A · kind A · utility
57Cited by
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15Claims
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Assignee
Inventor
Key dates
| Filing date | Aug 1, 1994 |
| Grant date | Jun 11, 1996 |
| Priority date | — |
| Expiry date | Aug 1, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2760/10122
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
Method for determining target site accessibility for a ribozyme by providing a DNA oligonucleotide having a nucleotide sequence complementary to the RNA target. The DNA oligonucleotide is contacted with the RNA target in the presence of an agent, such as RNAseH, such that the RNAseH cleaves the RNA target when the DNA and RNA form a duplex. The occurrence of cleavage is detected by any standard methodology. Those DNA oligonucleotides which provide a detectable level of cleavage indicate that the RNA target site is accessible to ribozyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.