Human liver epithelial cell line and culture media therefor
US5529920A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 1, 1992 |
| Grant date | Jun 25, 1996 |
| Priority date | — |
| Expiry date | May 1, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/914
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to long term multiplication and permanent establishment of a cell line of human liver epithelial cells(hepatocytes). The human liver epithelial cell line is capable of mitotically proliferating and continuously growing in vitro under suitable environmental conditions in suitable culture media. A method of producing an immortalized human liver epithelial cell line is also disclosed. The invention also relates to serum-free cell medium developed to support long term multiplication and permanent establishment of a cell line of human liver epithelial cells. The medium may contain an effective cell growth promoting amount of calcium ions; an effective cell growth promoting amount of glucose; an effective amount of insulin to aid cells in glucose uptake; an effective cell growth promoting amount of hydrocortisone; an effective amount of epidermal growth factor to bind epidermal growth factor receptors on cells; an effective amount of transferrin to increase DNA synthesis in cells; an effective amount of cholera toxin to increase DNA synthesis in cells; an effective amount of triiodothyronine to increase DNA synthesis in cells; and an effective growth promoti…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.