Patent · US Expired

Light scattering measurement cell for very small volumes

US5530540A · kind A · utility

23Cited by
6References
17Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 3, 1994
Grant dateJun 25, 1996
Priority date
Expiry dateAug 3, 2014

Classification

  • Technology area (CPC G)Physics
  • CPC primaryG01N2021/0346
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A modified light scattering cell, and associated method, whereby an eluant of very small dimension transverse to its direction of flow is entrained successively by two sheath flows and presented to a fine light beam that illuminates the entrained eluant as it flows through the light beam. The light scattered by the entrained eluant is collected by detectors outside of a transparent flow cell enveloping the sheath flow entrained eluant. The windows of the transparent flow cell through which the light beam enters and leaves are far removed from the scattering eluant and kept clear of eluant-contained particles by means of flow components that will form subsequently one of the eluant sheath flows employed. The eluant source is typically from a fine capillary such as found in capillary electrophoresis, capillary hydrodynamic fractionation, and flow cytometry applications.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.