Preparative two dimensional gel electrophoresis system

US5534121A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• B. Alex Merrick · Cary, US
• Rachel M. Patterson · Chapel Hill, US
• Robert Hall · Bear, US
• Chaoying He · Durham, US
• James Selkirk · Chapel Hill, US

Key dates

• Filing date: May 16, 1994
• Grant date: Jul 9, 1996
• Expiry date: May 16, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N27/44773
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A preparative two dimensional gel electrophoresis system which serves as a single procedure for separation and isolation of preparative amounts of proteins from complex biological preparations. The system includes sized-up isoelectric focusing tube gels and slab gel molds which allow for sample loads of between about 0.5 and 2 mg or greater. Increased protein loads, resolution and electrotransfer allow for subsequent sequencing of separated proteins by conventional methods.