Process for the synthesis of ribonucleic acid (RNA) using a novel deprotection reagent
US5552539A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jul 26, 1994 |
| Grant date | Sep 3, 1996 |
| Priority date | — |
| Expiry date | Jul 26, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02P20/55
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Process for RNA synthesis consisting in successively condensing necleosides for the formula (1) in which R.sup.1 is a puric pyrimidic optionally protected base, R.sup.2 is a leaving group such as the dimethoxytrityl radical, R.sup.3 is a phosphorus-containing radical, for example, phosphoramidite and R.sup.4 is a trialkylsilyl radical. The RNA obtained is then subjected to a protective treatment with a reagent of the formula (II), for example N(C.sub.2 H.sub.5).sub.3,3HF, to eliminate R.sup.4 protective groups of the 2' hydroxyl groups. Such reagent provides full deprotection in about ten hours, with an RNA of great length, for example, having more than 50 bases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.