Nucleic acid amplification process
US5554517A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 10, 1995 |
| Grant date | Sep 10, 1996 |
| Priority date | — |
| Expiry date | Feb 10, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/689
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a process for amplifying a specific nucleic acid sequence. The process involves synthesizing single-stranded RNA, single-stranded DNA and double-stranded DNA. The single-stranded RNA is a first template for a first primer, the single-stranded DNA is a second template for a second primer, and the double stranded DNA is a third template for synthesis of a plurality of copies of the first template. A sequence of the first primer or the second primer is complementary to a sequence of the specific nucleic acid and a sequence of the first primer or the second primer is homologous to a sequence of the specific nucleic acid. The amplification process may be used to increase the quantity of the specific nucleic acid sequence to allow detection, or to increase the purity of the specific nucleic acid sequence as a substitute for conventional cloning methodology.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.