Monoclonal antibody recognizing a surface molecule on a subset of antigen-stimulated T cells and on certain malignancies of T and B cell origin

US5556947A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Glenn H. Bock · Bethesda, US
• David L. Nelson · Bethesda, US
• Carole C. Kurman · Potomac, US
• Thomas A. Fleisher · Bethesda, US

Key dates

• Filing date: Aug 9, 1994
• Grant date: Sep 17, 1996
• Expiry date: Aug 9, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2317/73
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides an IL-6 dependent B-cell lymphoma cell line designated DS-1 deposited with the American Type Culture Collection, wherein the cell line is reactive with a monoclonal antibody produced by a hybridoma cell line designated 10D2F6 deposited with the American Type Culture Collection. The invention also provides a purified antigen reactive with a monoclonal antibody produced by 10D2F6. The antigen also exists on DS-1. Also provided is a method of detecting the presence of an antigen-stimulated T-cell comprising detecting the presence of the antigen on a lymphocyte. In addition, the invention provides a method of detecting the presence of a neoplastic cell comprising detecting the presence of the antigen on a cell which is not normal a T-cell.