Separation media for electrophoresis
US5569364A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 10, 1994 |
| Grant date | Oct 29, 1996 |
| Priority date | — |
| Expiry date | May 10, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S516/922
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Separation media for electrophoresis, and methods of filling and flushing of electrophoretic devices such as capillaries are described. By preparing submicron to above-micron sized cross-linked gel particles and using gel swelling equilibrium concepts, such devices can be easily filled and flushed. Gel particles can be prepared by inverse suspension, precipitation and suspension polymerization. These particles can be swollen and collapsed by small changes in temperature, pH, and ionic strength of solvent. Other approaches involve the formation of reversible cross-links by use of polyelectrolyte complexes, chelating agents or copolymers of hydrophobic and hydrophilic repeat units. Finally, reversibly solubilized systems may be used to change the viscosity of the media.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.