Bispecific and oligospecific mono-and oligovalent receptors, the preparation and use thereof

US5591828A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Klaus Bosslet · Marburg, DE
• Peter Hermentin · Marburg, DE
• Gerhard Seemann · Lahntal, DE
• Ludwig Kuhlmann · Flörsheim, DE
• Axel Steinstr asser · Liederbach, DE

Key dates

• Filing date: Sep 29, 1994
• Grant date: Jan 7, 1997
• Expiry date: Sep 29, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K38/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention relates to bispecific and oligospecific, mono- and oligovalent receptors which are prepared by gene manipulation by fusion of DNA coding for F(ab) fragments of antibodies of two or more different specificities by means of suitable linkers. In this connection, one specificity is preferably directed either against an epitope, which is located on the cell membrane or in the interstitium, of a tumor-associated antigen (TAA) or against an epitope in the tumor endothelium (TE), while the other specificities relate to high-molecular or low-molecular weight ligands and react, for example, with the Komplexons ethylenediaminetetraacetate and diethylenetriaminepentaacetate in Y90 complexed form (EDTA-Y90 and DTPA-Y90 respectively). In a particularly preferred embodiment, the binding with the Komplexons takes place on the Komplexon receptor arm via fos-jun interaction (or else avidin-biotin interaction). Other preferred specificities have catalytic properties.