Patent · US Expired

Recombinant methods of making gamma interferon compositions

US5595888A · kind A · utility

51Cited by
14References
5Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 24, 1994
Grant dateJan 21, 1997
Priority date—
Expiry dateMar 24, 2014

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S930/30
  • WIPO fieldPharmaceuticals
  • WIPO sectorChemistry

Abstract

Provided are methods for the production of mixtures of interferon (IFN)-.gamma. polypeptides. The methods employ a single species of IFN-.sub..gamma. DNA having codons for Met-Gln and for residues 2-143 of the mature human IFN-.sub..gamma. amino acid sequence. Expression of such DNA in a suitable recombinant host cell affords a mixture of polypeptides having N-terminal Met-Gln, H-Gln, or H-pyroglutamate residues and C-termini at Arg.sup.139, Ser.sup.142, or Gln.sup.143 of the mature IFN-.sub..gamma. sequence. The polypeptides so produced exhibit the antiviral and antiproliferative activities as well as the acid lability characteristic of native human IFN-.sub..gamma..

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.