Single chain B3 antibody fusion proteins and their uses

US5608039A · kind A · utility

Assignee

• The United States of America as Represented by the Secretary of the Department of Health and Human Services · US

Inventors

• Ira H. Pastan · Potomac, US
• Mark C. Willingham · Summerville, US
• David Fitzgerald · Rockville, US
• Ulrich Brinkmann · Weilheim, DE
• Lee Pai · Westfield, US

Key dates

• Filing date: Oct 28, 1994
• Grant date: Mar 4, 1997
• Expiry date: Oct 28, 2014

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/867
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

This invention provides for recombinant single chain antibodies capable of specifically binding to a Lewis.sup.Y -related carbohydrate antigen and fusion proteins comprising these antibodies. More particularly, the invention provides for single chain Fv regions of the monoclonal antibody B3. The invention also provides for a method of improving the binding affinity of antibodies lacking a serine at position 95 of the V.sub.H region that involves mutating position 95 to a serine.