Pteridine nucletide analogs as fluorescent DNA probes

US5612468A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Mary E. Hawkins · Potomac, US
• Wolfgang Pfleiderer · Konstanz, DE
• Michael D. Davis · Laurel, US
• Frank Balis · Bethesda, US

Key dates

• Filing date: May 26, 1995
• Grant date: Mar 18, 1997
• Expiry date: May 26, 2015

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07H19/22
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

The invention provides novel pteridine nucleotides which are highly fluorescent under physiological conditions and which may be used in the chemical synthesis of fluorescent oligonucleotides. The invention further provides for fluorescent oligonucleotides comprising one or more pteridine nucleotides. In addition the invention provides for pteridine nucleotide triphosphates which may be used as the constituent monomers in DNA amplification procedures.