Methods for determining the presence of functional p53 in mammalian cells

US5616463A · kind A · utility

Assignees

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US
• THE JOHNS HOPKINS UNIVERSITY · US

Inventors

• Albert J. Fornace, Jr. · Bethesda, US
• Michael Kastan · Cordova, US

Key dates

• Filing date: Aug 10, 1994
• Grant date: Apr 1, 1997
• Expiry date: Aug 10, 2014

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/47
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The dependence of ionizing radiation-induced GADD45 mRNA expression on the presence of functional p53 in mammalian cells is disclosed. First and second oligonucleotide sequences are provided which can form a double-stranded oligomer capable of binding to functional p53 protein. The present invention demonstrates that the dependence of ionizing radiation-induced GADD45 mRNA expression on the presence of functional p53 and the binding of functional p53 to a double-stranded oligomer binding sequence can serve as the basis for methods for determining the presence of functional p53 in mammalian cell lines and tumors.