Solution phase nucleic acid sandwich assays having reduced background noise
US5635352A · kind A · utility
361Cited by
7References
25Claims
0Family size
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Key dates
| Filing date | Apr 26, 1995 |
| Grant date | Jun 3, 1997 |
| Priority date | — |
| Expiry date | Apr 26, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
New techniques are provided for substantially reducing background signals encountered in solution phase hybridization assays. The techniques are premised on eliminating or significantly reducing the phenomena of nonspecific hybridization and nonspecific binding, so as to provide a detectable signal which is produced only in the presence of the target polynucleotide of interest. In certain embodiments, methods are provided for increasing the signal which can otherwise be diminished in noise reduction. Kits for carrying out the novel assays are provided as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.