Method of mobilizing pluripotential hematopoietic stem cells with IL-7

US5637323A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Robert Wiltrout · Woodsboro, US
• Francis W. Ruscetti · New Market, US
• Krzysztof J. Grzegorzewski · Montgomery Village, US
• Jonathan R. Keller · Frederick, US
• Kristin L. Komschlies-McConville · Westminister, US

Key dates

• Filing date: Nov 16, 1994
• Grant date: Jun 10, 1997
• Expiry date: Nov 16, 2014

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2035/124
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

This invention provides a method of isolating an increased number of hematopoietic stem cells from a subject comprising a) administering interleukin-7 to the subject in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; and, b) isolating a population of leukocytes enriched for hematopoietic stem cells from the peripheral blood. Also provided is a method of transplanting an increased number of hematopoietic stem cells from a donor to a recipient to enhance repopulation of the recipient's hematopoietic and immune cells comprising a) administering interleukin-7 to the donor in an amount that mobilizes the hematopoietic stem cells to the peripheral blood; b) isolating a population of leukocytes enriched for hematopoietic stem cells from the donor's peripheral blood; and, c) transplanting the isolated population of leukocytes enriched for hematopoietic stem cells to the recipient, thereby enhancing the repopulation of the recipient's hematopoietic and immune cells. Finally, the invention provides a method of improving engraftment of a bone marrow transplant or a peripheral blood leukocyte transplant from a donor to a recipient comprising administering interle…