Method to detect protein-protein interactions
US5637463A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 4, 1995 |
| Grant date | Jun 10, 1997 |
| Priority date | — |
| Expiry date | May 4, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6897
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for studying protein-protein interactions which require posttranslational modification of one of the proteins. The interaction is detected by reconstituting the activity of a transcriptional activator. This activity is dependent on the interactions between three different proteins. These include two chimeric proteins, one of which must be posttranslationally modified by the activity of the third protein in order for the chimeric proteins to interact. One of the chimeric proteins contains a transcriptional activation domain fused to a test protein. The second chimeric protein contains a DNA-binding domain of a transcriptional activator fused to the other test protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.