Process for bacterial production of polypeptides
US5639635A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 3, 1994 |
| Grant date | Jun 17, 1997 |
| Priority date | — |
| Expiry date | Nov 3, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A process is provided for producing a heterologous polypeptide in bacteria, which process comprises: PA1 (a) culturing bacterial cells, which cells comprise nucleic acid encoding a DsbA or DsbC protein, nucleic acid encoding the heterologous polypeptide, a signal sequence for secretion of both the DsbA or DsbC protein and the heterologous polypeptide, and an inducible promoter for both the nucleic acid encoding the DsbA or DsbC protein and the nucleic acid encoding the heterologous polypeptide, under conditions whereby expression of the nucleic acid encoding the DsbA or DsbC protein is induced prior to induction of the expression of the nucleic acid encoding the heterologous polypeptide, and under conditions whereby either both the heterologous polypeptide and the DsbA or DsbC protein are secreted into the periplasm of the bacteria or the heterologous polypeptide is secreted into the medium in which the bacterial cells are cultured; and PA1 (b) recovering the heterologous polypeptide from the periplasm or the culture medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.