Detection of a genetic locus encoding resistance to rifampin in mycobacterial cultures and in clinical specimens
US5643723A · kind A · utility
22Cited by
6References
23Claims
0Family size
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Key dates
| Filing date | May 26, 1994 |
| Grant date | Jul 1, 1997 |
| Priority date | — |
| Expiry date | May 26, 2014 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/863
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is provided for detecting M. tuberculosis or mutants thereof, particularly rifampin-resistant MTB, in a biological sample comprising: isolating DNA from the biological sample; amplifying the isolated DNA under hybridizing conditions with a primer set that targets portions of the gene encoding rpoB; wherein the pimer set comprises at least one primer that hybridizes under hybridizing conditions to at least one signature nucleotide for M. tuberculosis; and isolating and sequencing the amplified DNA to determine the presence or absence of M. tuberculosis or mutants thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.