Use of pairs of leucine zipper peptides in immunoassay methods

US5643731A · kind A · utility

Assignee

• Behringwerke Aktiengesellschaft · DE

Inventors

• Klaus Bosslet · Marburg, DE
• Peter Hermentin · Marburg, DE
• Hans-Harald Sedlacek · Marburg, DE
• Bernhard Auerbach · Marburg, DE
• Peter Pfleiderer · Marburg, DE
• Rolf Muller · Marburg, DE

Key dates

• Filing date: Jun 6, 1995
• Grant date: Jul 1, 1997
• Expiry date: Jun 6, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/824
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention relates to a method of using a pair of leucine zipper peptides for in vitro diagnosis, in particular, for the immunochemical detection and determination of an analyte in a biological liquid. In one method, the first leucine zipper peptide is immobilized by attaching it to a solid support, the second leucine zipper peptide is coupled to a specific binding partner for the analyte, the two peptides are brought into contact, the sample of the biological liquid is brought into contact with the immobilized first peptide and the specific binding partner for the analyte, and the amount of analyte bound to the binding partner is determined. The leucine zipper peptides are preferably v-fos and c-jun.