Expression of functional antibody fragments
US5648237A · kind A · utility
468Cited by
5References
47Claims
0Family size
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Key dates
| Filing date | May 3, 1995 |
| Grant date | Jul 15, 1997 |
| Priority date | — |
| Expiry date | May 3, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/972
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the high yield production of antibody Fv-containing polypeptides, especially Fab' and F(ab').sub.2 antibody fragments are provided. Expression of heavy and light chain Fv in a microbial secretory system is followed by recovery of Fv from the periplasm under conditions that maintain a cysteine residue as a free thiol. The free thiol is reacted with free thiol of an antibody fragment of the same or differing specificity, or with agents such as diagnostic labels or therapeutic moieties. The products offer advantages of homogeneity and purity not available through the use of known methods for preparing such derivatives.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.