Refolding of misfolded insulin-like growth factor-I
US5663304A · kind A · utility
27Cited by
21References
12Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 20, 1993 |
| Grant date | Sep 2, 1997 |
| Priority date | — |
| Expiry date | Aug 20, 2013 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A composition is provided comprising about 0.1 to 15 mg/mL of a polypeptide in a buffer having a pH of about 7-12 comprising about 5-40% (v/v) of an alcoholic or polar aprotic solvent, about 0.2 to 3M of an alkaline earth, alkali metal, or ammonium salt, about 0.1 to 9M of a chaotropic agent, and about 0.01 to 15 .mu.M of a copper or manganese salt. The buffer is suitably used in a method for refolding improperly folded polypeptides.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.