Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor
US5681697A · kind A · utility
123Cited by
7References
8Claims
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Key dates
| Filing date | Dec 8, 1993 |
| Grant date | Oct 28, 1997 |
| Priority date | — |
| Expiry date | Dec 8, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
New techniques are provided for substantially reducing background signals encountered in solution phase hybridization assays. The techniques are premised on eliminating or significantly reducing the phenomena of nonspecific hybridization and nonspecific binding, so as to provide a detectable signal which is produced only in the presence of the target polynucleotide of interest. In certain embodiments, methods are provided for increasing the signal which can otherwise be diminished in noise reduction. Kits for carrying out the novel assays are provided as well.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.