Reduction of nonspecific hybridization by using novel base-pairing schemes
US5681702A · kind A · utility
169Cited by
2References
8Claims
0Family size
Assignee
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Key dates
| Filing date | Aug 30, 1994 |
| Grant date | Oct 28, 1997 |
| Priority date | — |
| Expiry date | Aug 30, 2014 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/336
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are provided for substantially reducing background signals encountered in nucleic acid hybridization assays. The method is premised on the elimination or significant reduction of the phenomenon of nonspecific hybridization, so as to provide a detectable signal which is produced only in the presence the target polynucleotide of interest. In addition, a novel method for the chemical synthesis of isoguanosine or 2'-deoxy-isoguanosine is provided. The invention also has applications in antisense and aptamer therapeutics and drug discovery.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.