Systematic evolution of ligands by exponential enrichment: blended SELEX
US5683867A · kind A · utility
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16Claims
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Key dates
| Filing date | Apr 28, 1994 |
| Grant date | Nov 4, 1997 |
| Priority date | — |
| Expiry date | Apr 28, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/976
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl (SEQ ID NO:2) ketone. This elastase blended nucleic acid ligand inhibits the biological activity of elastase.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.