Growth hormone variants with altered binding properties
US5688666A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 14, 1994 |
| Grant date | Nov 18, 1997 |
| Priority date | — |
| Expiry date | Jan 14, 2014 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/61
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for selecting novel proteins such as growth hormone variants having altered binding properties for a growth hormone receptor molecule is provided. The method comprises fusing a gene encoding a hormone to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disposed are preferred phagemid expression vectors and selected human growth hormone variants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.