Aqueous multiple-phase isolation of polypeptide
US5695958A · kind A · utility
5Cited by
18References
25Claims
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Key dates
| Filing date | May 17, 1995 |
| Grant date | Dec 9, 1997 |
| Priority date | — |
| Expiry date | May 17, 2015 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S530/808
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method is described for isolating an exogenous polypeptide in a non-native conformation from cells, such as an aqueous fermentation broth, in which it is prepared comprising contacting the polypeptide with a chaotropic agent and preferably a reducing agent and with phase-forming species to form multiple aqueous phases, with one of the phases being enriched in the polypeptide and depleted in the biomass solids and nucleic acids originating from the cells. Preferably, the method results in two aqueous phases, with the upper phase being enriched in the polypeptide.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.