Purification of plasmid DNA during column chromatography
US5707812A · kind A · utility
40Cited by
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11Claims
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Key dates
| Filing date | Aug 6, 1996 |
| Grant date | Jan 13, 1998 |
| Priority date | — |
| Expiry date | Aug 6, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for purifying plasmid DNA during column chromatography is provided. A short chain polymeric alcohol, preferably polyethylene glycol, or another DNA condensation agent, is added to a DNA sample prior to column chromatography. The short chain polymeric alcohol or condensation agent promotes improved isolation of plasmid DNA and may be used for large scale purification, particularly for manufacturing plasmid DNA as a biopharmaceutical.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.