Process for immobilizing nucleic acid probes on polystyrene surfaces
US5712383A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 10, 1995 |
| Grant date | Jan 27, 1998 |
| Priority date | — |
| Expiry date | May 10, 2015 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6834
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Nucleic acid probes are immobilized on polystyrene surfaces such as the wells of microtiter plates for use in solution phase nucleic acid sandwich hybridization assays, particularly those using large branched DNA amplification multimers, by: (a) cleansing the surface by washing it sequentially with a strong acid, a strong base, and water, (b) passively adsorbing a polypeptide having primary amino groups onto the cleansed surface, and (c) covalently bonding the probe to the adsorbed polypeptide via a base-stable bifunctional crosslinking agent, and (d) subjecting the surface to conditions that simulate the hybridization conditions used in the assay.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.