Recombinant DNA and transformant containing the same
US5731193A · kind A · utility
23Cited by
10References
16Claims
0Family size
Assignee
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Key dates
| Filing date | Mar 31, 1997 |
| Grant date | Mar 24, 1998 |
| Priority date | — |
| Expiry date | Mar 31, 2017 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2830/85
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Insertion of IFN-alpha promoters in recombinant DNAs improves their expression efficiencies for useful polypeptides. Expression of such a recombinant DNA in host cells of mammalian origin is artificially controllable by the presence and absence of external stimuli using IFN-alpha inducers. Thus, transformants with such a recombinant DNA readily increase to a maximized cell density with causing neither damages nor extinction due to polypeptides they produce, and subsequent exposure to IFN-alpha inducers allows the proliferated cells to efficiently produce polypeptides with significant glycosylations.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.