Enrichment method for variant proteins having altered binding properties, M13 phagemids, and growth hormone variants
US5750373A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Apr 30, 1993 |
| Grant date | May 12, 1998 |
| Priority date | — |
| Expiry date | Apr 30, 2013 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/12
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.