Inhibition of cell proliferation using antisense oligonucleotides

US5756476A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Stephen Epstein · Rockville, US
• Edith Speir · Annandale, US
• Ellis F. Unger · Silver Spring, US

Key dates

• Filing date: Jan 26, 1994
• Grant date: May 26, 1998
• Expiry date: Jan 26, 2014

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2799/021
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The present invention provides a method of inhibiting restenosis of a blood vessel in a mammal after mechanical treatment of the vessel to reduce a stenosis. The method includes contacting proliferating smooth muscle cells in the vessel with an antisense oligonucleotide directed against a cellular division cycle gene product. This gene product can be one of the following: c-myc, PCNA, and cyclin B.sub.1. The antisense oligonucleotide is used in an amount effective to inhibit translation of the cellular division cycle gene product in the cell.