Method and compositions for making ACSF and ACSF antagonists

US5763195A · kind A · utility

Assignees

• Genentech, Inc. · US
• The University of Melbourne · AU

Inventors

• William I. Wood · Hillsborough, US
• Thomas Martin · Konstanz, DE
• Larry Suva · Little Rock, US

Key dates

• Filing date: Apr 20, 1995
• Grant date: Jun 9, 1998
• Expiry date: Apr 20, 2015

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/04
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The complete amino acid and nucleotide sequence for adenylate cyclase stimulating factor is provided, thereby facilitating the synthesis of ACSF in recombinant cell culture. ACSF amino acid sequence variants and ACSF antibodies are provided which are useful in the treatment of humoral hypercalcemia of malignancy or in immunoassays for ACSF. In particular, antibodies capable of binding only the C-terminal domains of ACSF are useful in immunoassays for ACSF which avoid interference by parathyroid hormone. Also provided are novel polypeptides selected from the group of the ACSF basic peptide, the ACSF C-terminal peptide, or the ACSF domain containing both of the basic and C-terminal peptides.