Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US5773258A · kind A · utility
192Cited by
2References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 11, 1996 |
| Grant date | Jun 30, 1998 |
| Priority date | — |
| Expiry date | Jul 11, 2016 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides methods for the amplification of nucleic acids using a reversibly inactivated thermostable enzyme. The reversibly inactivated enzyme is the result of a chemical modification of the protein which inactivates the enzyme. The activity of the inactivated enzyme is recovered by an incubation of the reaction mixture at an elevated temperature prior to, or as part of, the amplification reaction. Non-specific amplification is reduced because the reaction mixture does not support the formation of extension products prior to the activating incubation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.