Methods of determining the structure of a compound encoded by identifiers having tags

US5789172A · kind A · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• Cold Spring Harbor Laboratory · US

Inventors

• W. Clark Still · New York, US
• Michael Wigler · Lloyd Harbor, US
• Michael H. J. Ohlmeyer · Plainsboro, US
• Lawrence W. Dillard · Hopewell, US
• John Charles Reader · Princeton, US

Key dates

• Filing date: Jul 11, 1996
• Grant date: Aug 4, 1998
• Expiry date: Jul 11, 2016

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B70/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Encoded combinatorial chemistry is provided, where sequential synthetic schemes are recorded using organic molecules, which define choice of reactant, and stage, as the same or different bit of information. Various products can be produced in the multi-stage synthesis, such as oligomers and synthetic non-repetitive organic molecules. Conveniently, nested families of compounds can be employed as identifiers, where number and/or position of a substituent define the choice. Alternatively, detectable functionalities may be employed, such as radioisotopes, fluorescers, halogens, and the like, where presence and ratios of two different groups can be used to define stage or choice. Particularly, pluralities of identifiers may be used to provide a binary or higher code, so as to define a plurality of choices with only a few detachable tags. The particles may be screened for a characteristic of interest, particularly binding affinity, where the products may be detached from the particle or retained on the particle. The reaction history of the particles which are positive for the characteristic can be determined by the release of the tags and analysis to define the reaction history of the pa…