Methods for producing and analyzing biopolymer ladders

US5792664A · kind A · utility

Assignees

• The Rockefeller University · US
• THE SCRIPPS RESEARCH INSTITUTE · US
• Ciphergen Biosystems, Inc. · US

Inventors

• Brian T. Chait · New York, US
• Rong Wang · Mianyang, CN
• Stephen B. H. Kent · Melbourne, AU
• Steven M. Clark · Springfield, US

Key dates

• Filing date: Jun 7, 1995
• Grant date: Aug 11, 1998
• Expiry date: Jun 7, 2015

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10T436/24
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods of producing biopolymer ladders and their use to obtain structural information about the biopolymer. The ladders are produced by setting up catalytic cleavage and terminating reactions at the end of biopolymer molecules. The terminating reactions terminate cleavage of a percentage of the biopolymer molecules at each round of cleavage.