Sequencing DNA; a modification of the polymerase chain reaction

US5817797A · kind A · utility

Assignee

• The United States of America, as represented by the Department of Health and Human Services · US

Inventors

• Lloyd G. Mitchell · Durham, US
• Carl R. Merril · Rockville, US

Key dates

• Filing date: Jun 1, 1988
• Grant date: Oct 6, 1998
• Expiry date: Jun 1, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention provides a process wherein a biotinylated oligonucleotide primer and an oligonucleotide primer which has not undergone biotinylation are used when amplifying a DNA sequence to facilitate separation of the DNA strands following the polymerase chain reaction process. The biotinylation/PCR product is then exposed to a support which will selectively bind the biotinylated strand to allow selective elution of the product.