Partially double-stranded oligonucleotide and method for forming oligonucleotide
US5830643A · kind A · utility
43Cited by
9References
5Claims
0Family size
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Key dates
| Filing date | Nov 10, 1992 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | Nov 10, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for forming an oligonucleotide comprises the steps of synthesizing a pair of oligonucleotides, one oligonucleotide having a base sequence portion comlementary to that of the other at a part of the terminal end region thereof; binding the pair of oligonucleotides at the complementary base sequence portions; and polymerizing nucleic acid bases onto the resulting pair of oligonuceotides. The method may further comprise the step of separating the oligonucleotides thus obtained into single strands.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.