DNA mutagenesis by random fragmentation and reassembly
US5830721A · kind A · utility
778Cited by
8References
28Claims
0Family size
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Key dates
| Filing date | Mar 4, 1996 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | Mar 4, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/622
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for generating libraries of displayed peptides and/or antibodies (Abs) suitable for affinity interaction screening or phenotypic screening comprising: (i) obtaining selected library members comprising a displayed peptide and/or Ab and the corressponding polynucleotide (PN), or copies of it, (ii) pooling and fragmenting the PN, or copies of it, to form fragments, (iii) performing PCR amplification and thereby homologously recombining the fragments to form a shuffled pool of recombined PNs, which are not present in the selected library of (i).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.