I Sce I-induced gene replacement and gene conversion in embryonic stem cells
US5830729A · kind A · utility
34Cited by
1References
8Claims
0Family size
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Key dates
| Filing date | Aug 7, 1996 |
| Grant date | Nov 3, 1998 |
| Priority date | — |
| Expiry date | Aug 7, 2016 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/4735
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to a method of constructing a villin gene hybrid by inserting an I-Sce I restriction site next to or within a gene or cDNA encoding a villin protein. The insertion site of the I-Sce I restriction site is chosen as to provide a first downstream part and a second upstream part from the site, containing at least twelve nucleotides of the gene or cDNA encoding the villin protein. Furthermore, the insertion of the restriction permits a high frequency of homologous recombination events. The villin gene hybrid may be used to transfect eukaryotic cells, and particularly, embryonic stem cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.