Patent · US Expired

Human growth hormone variants

US5834598A · kind A · utility

65Cited by
3References
5Claims
0Family size

Assignee

Inventors

Key dates

Filing dateJun 5, 1995
Grant dateNov 10, 1998
Priority date
Expiry dateJun 5, 2015

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S930/12
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

A method for selecting novel proteins such as growth hormone and antibody fragment variants having altered binding properties for their respective receptor molecules is provided. The method comprises fusing a gene encoding a protein of interest to the carboxy terminal domain of the gene III coat protein of the filamentous phage M13. The gene fusion is mutated to form a library of structurally related fusion proteins that are expressed in low quantity on the surface of a phagemid particle. Biological selection and screening are employed to identify novel ligands useful as drug candidates. Disclosed are preferred phagemid expression vectors and selected human growth hormone variants.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.